عرض تفاصيل البحث

ادناه جميع التفاصيل الخاصة بالبحث المطلوب عرضه. توفر هذه المنصة معلومات اساسية حول البحث, لمزيد من التفاصيل يرجى متابعة التصفح من خلال الضغط على الرابط الاصلي للبحث او رابط DOI.

عنوان البحث
Effect Of Dark / Light Daytime Cycle On Integrin Protein Expression In Retina Of Adult Mice
عنوان المجلة
Indian Journal Of Natural Sciences
ISSN-0976-0997
تفاصيل النشر
سنة النشر - 2019 / الفهرس الاصلي للمجلة - 10 : 57 (عدد الصفحات 14)
تصنيف البحث
anatomy and histology - المجموعة الطبية
رابط DOI
#
رابط البحث
فتح في موقع المجلة الرسمي
البحث والاستدامة
غير مرتبط باهداف التنمية المستدامة  
البحث والمجتمع
نعم , يدعم
الكلمات المفتاحية
retina ,integrin, photoreceptors

اسم الباحثجهة الانتساببلد الباحث
Ali Abdul Sattar University of Sumer Iraq
Haider Jaafer University of Alnahrain Iraq
Hussein Abaas University of Alnahrain Iraq

The retina is a thin, delicate, transparent sheet of tissue derived from the neuroectoderm. It comprises the sensory neurons that begin the visual pathway. Light must traverse these many layers before initiating signal transduction in the rods and cones. Integrins are a family of heterodimer plasma membrane proteins important for cell-cell and cell-extracellular matrix of retina.They consist of variable and subunits. It provides a link between their extracellular ligand and the cytoskeleton of sensory neurons and helps modulate various signaling pathways including, cell adhesion, migration, differentiation, angiogenesis.Aim of this study is to evaluate the morphological and histological changes of retina in response to dark and light cycle. To assess the IHC reactivity of integrin protein marker in retina in response to dark and light cycle by applying Aperio image scope analysis software. A forty (40) adult healthy male mice (albino mice), weighing between 25-30 g were divided into four groups (each with 10 mice): A, B, C &D according to dark/ light day periods. Animals in group A were kept in normal diurnal variance, 10hr dark and 14 hr light. Group B animals were kept in full time light. Group C animals were kept in full dark. Group D animals were kept in inverse state (10hr of full dark during daylight and 14hr light during the night). All animals wereeuthanizedat 45 days by inhalation of chloroform in soaked cotton piece. The whole eye ball was dissected and fixed in 10% natural buffered formalin, processed for paraffin blocks and stained for H&Eand IHCfor integrin (monoclonal antibodies Ab183666). Histological slides were assessed by image J softwere version (1.47p) for histomorphmetric analysis of thickness of retina, rods and cons, and bipolar cells .Immunohistochemical analysis done by Aperio positive pixel count algorithm (V11) for assessment of total positivity of integrin. Histomorphometric analysis revealed that the highest thickness of retina was recorded in dark group (139.02±2.53 µm), while control group had the least thickness (116.86± 6.67 µm) with significant differences. Mean bipolar cells thickness ranged from 70.71±4.81 µm in control group to 97.21±8.17 µm in dark group. In between, lies bipolar cell thickness of inverse and light group with significant differences. The highest and least thickness of rods and cons were reported in dark group and control group respectively (80.33±5.06 µm and 43.46 µm
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